Philippine Local Forages as Sustainable Feed Alternative for Dairy Cattle

 


 

Conclusions

We successfully reintroduced the six select forages in selected areas in Batangas and Siquijor through the research and extension/training components of the project in collaboration with our project partners, BatStateU, De La Salle Lipa, PABRD, Benacorn Corp. PVO Siquijor and SAMABACO.   The collaboration with Batangas State University Lobo Campus allowed us to conduct forage performance evaluation, establish a duplicate forage genebank and conduct a trainor’s training on forage production and conservation, activities which will surely have an impact in the revival of interest on local pasture resources.  BatStateU can replicate the training in their area by using the training module and manual we developed for their use in educating the small farmers on the importance of quality forages as cheap source of feed. 

The forage introduction in Siquijor in collaboration with the Provincial Veterinary Office of Siquijor also provided strong support system to the cattle genomic project which tapped the local cattle population of the island as potential source of a local dairy breed in the country.  

A tissue culture laboratory facility at DTRI-UPLB was established for the micropropagation of hybrid Napier grass. The successful propagation of Napier grass from leaf tissues via tissue culture was a pioneering work in the Philippines. This accomplishment will have important applications with regards to rapid multiplication of planting materials from the limited samples of elite forages. The availability of enough quantity of planting materials is vital in support of the pasture development initiatives of both public and private sectors to sustain the growing ruminant industry.

The forage performance evaluation conducted in three locations affirmed the significant effect of environment i.e. soil and climate on the herbage yield of the six select forages.   The genetic potential of forages to produce high biomass was hindered by the unfavorable environment e.g. infertile soil, lack of water in the location where these were introduced. Long established concept of genetic x environment interaction on crop performance would necessitate the application of location –specific cultural management interventions to optimize the performance the select forages.  In the present study, the same cultural management practices such as fertilizer application was applied in all three locations to show the genotypic differences among the six forage species as expressed phenotypically through parameters like plant height, herbage yield and nutrient composition.

The TMR feeding study provided results that lean towards the claimed advantage of feeding this complete dairy ration to milking cows. More milk of better quality i.e. high protein, minerals and total solids  was produced by cows fed the more nutritious TMR (HD-TMR) compared with the high forage TMR (LD-TMR), however at the expense of lowered fat content. Higher number of experimental animals and replication of the feeding trial in backyard-, semi- and commercial- dairy farms would be necessary to obtain more valid results that would merit its commercial viability to dairy farmer-adopters.

The unexpected deterioration of RNA samples sent to the US for RNA sequencing was unexpected. The physiological effects that will be inferred through bioinformatics from the sequencing data of RNA extracted from milk of experimental animals was not determined in the present study. Nevertheless, milk yield and quality data obtained leaned towards the beneficial effects of feeding high quality ration such as the TMR at the animal production level.  We will consider pursuing this laboratory procedure in our future researches.

Nevertheless, the project generated science-based data and information from the numerous R & D activities conducted during its 15 months duration that could encourage our dairy farmers to reconsider the sustainable use of the local forage resources that we currently have and explore the utilization of indigenous plants as feed source to sustain animal production.

 

September 1, 2016 to January 31, 2018. Source of Fund: USAID STRIDE & RTI

Agronomic Performance and Feeding Value of Mulato II and Mombasa Grasses in Dairy Cattle


Abstract

Mulato II (Brachiaria hybrid) and Mombasa (Panicum maximum Jacq. cv. Mombasa) were compared with Signal (Brachiaria decumbens) and Slender Guinea (Panicum maximum var. trichoglume), respectively in pure stand or when mixed with either Arachis (Arachis pintoi) or Centrosema (Centrosema molle) in terms of the herbage yield, nutrient composition, in vitro digestibility and persistence. Rumen degradation experiment was also conducted to estimate nutritive values as correlated with the gas production levels from incubated samples of the Mulato II and Mombasa grasses at different cutting intervals (Study 1). 

The two grasses were fed to growing heifers to evaluate the growth performance i.e. liveweight gain in comparison with Napier (Study 2).  A comparative feeding study was also conducted to compare the dry matter intake, milk yield and composition of Holstein Friesian x Sahiwal (HF x SH) cows fed with Mulato II and Mombasa in comparison with Napier (Study 3).

Laboratory experiments were carried out to evaluate the effects of two additives on the physical and chemical characteristics of the two grasses. Ensiled Mulato II and Mombasa was also fed to HF x SH cows to determine their influence on the DMI, milk yield and milk composition in comparison with those fed Napier silage (Study 4). Cost and return analysis was also conducted on their establishment, maintenance, conservation and utilization (Study 5).

Results showed the comparable performance of Mulato II and Mombasa with the close relative grasses in pure stand and when mixed with forage legumes. Feeding either of Mulato II or Mombasa resulted in similar liveweight gain of growing heifers. Milk yield and composition of cows fed fresh Mulato II and Mombasa were comparable with cows fed Napier grass. Higher DMI was noted in cows fed Mulato II silage (11 kg/day) and Mombasa silage (10.1 kg/day) compared with Napier (8.9 kg/day). Mulato II- and Mombasa- silage- fed cows produced an average daily milk of 8.93 kg and 8.83 kg respectively compared with 7.18 kg for cows fed Napier silage during the 60-day period.

Cost analysis showed that a kg of dry matter costs Php 1.84, Php 1.50 and Php 1.35 respectively for Mulato II, Mombasa and Napier. When ensiled, a kg of DM costs Php 15.45, Php 14.82 and 16.11, respectively for Mulato II, Mombasa and Napier.  Silage DM from Mombasa grass was cheaper than Mulato II and Napier because of the higher DM content (24%) of the former.

 

October 1, 2016 to April 30, 2019. Source of Fund: PCAARRD- DOST

DTRI, DA-BAR launches NIR Technology-based project

DTRI, UPLB – With the aim to have a fast, effective, and inexpensive feed analysis system, the project “Calibration and Optimization of Near Infrared Reflectance Spectroscopy for Nutrient Content Determination of Feedstuffs: A Tool for Reduced Feed Analyses Cost for Small-Hold Farmers and Government Research/Regulatory Institutions” was launched last September 3, 2019 at the Dairy Training and Research Institute (DTRI) Auditorium.. The project, funded by the Department of Agriculture’s Bureau of Agricultural Research (DA-BAR) is a collaboration of DTRI-UPLB, DA Regional Feed Laboratories, state universities, and other stakeholders that would benefit from the project. This project is just among the various projects being launched and conducted by DTRI in gearing towards modernization and collaborative dairy science research.

Those present in the project launch were Dr. Elpidio M. Agbisit J. (Dean, College of Agriculture and Food Science); Dr. Amado A. Angeles (Director, DTRI) and project staff; DA-BAR representatives: Marnelie Gadong-Subong, Jay Bermas, Rhea Desalesa, and Ian Panaga; and the UPLB Accounting Office represented by Joan Mendoza, Rhodora Violanta, and Ellen Asegurado. 

The results of the research project will enable feed manufacturers and users decide on what feed and feed ingredients they will use for their animals. This will also pave the way for farmers to afford services on identifying nutrient composition of their raw materials and complete feeds. Lastly, the study results will also benefit the different DA laboratories in the Philippines. 

 

 

NIRS project visits DA Feed Laboratories, SUCs

To forge a stronger collaboration among stakeholders, the Near-Infrared Reflectance Spectroscopy (NIRS) project team visited Department of Agriculture (DA) feed laboratories and State Universities and Colleges (SUCs) last December 17 to 19, 2020. This is part of the information dissemination strategies of the said project of DTRI on NIRS. Among those visited were the Regional Feed Chemical Analysis Laboratory of the DA (DA-RFCAL) in regions I, II, and III; Cagayan State University (CSU); and Central Luzon State University (CLSU). 

Apart from data accumulation from the laboratories, the project team conducted meetings with representatives from the above-mentioned institutions. In the meetings, DTRI director and project leader Dr. Amado A. Angeles seized the opportunity  to address the need to strengthen research, most especially in the dairy industry. Moreover, Dr. Angeles presented the need for faster and more affordable feed analysis services to cater farmers, students, and researchers to help them in the improvement of their respective fields. In turn, the visited laboratories and SUCs gave their full support for the project in the hopes to further cement contributions of their respected institutions to our agriculture.

The project team targets to visit DA-RFCAL and SCUs throughout the country for wider scope of collaboration and service.

 

PDRC Project 1 Team visits key AI and genetics facility in Australia

In the photo are (from L to R): Dr. Errol Jay Y. Balagan, CLSU; Mr. Rainie Rich Chucky S. Yambao, UPLB; Dr. Shirley S. Villanueva, USEP; Ms. Karla Joy S. Ty, UPLB; Mr. Anthony Shelly, Genetics Australia Chief Executive Officer; Dr. Amado A. Angeles, UPLB DTRI Director; Mr. Peter Thurn Genetics Australia Breeding, Genetics and Production Manager; Mr. Rob Derksen Genetics Australia Export Manager; and Dr. Juan S. Daquioag II, CSU. (Photo courtesy of Rob Derksen of Genetics Australia)

Six representatives from the Philippine Dairy Research Consortium (PDRC) Dairy Enhancement Program under Project 1 Application and Improvement of Embryo Transfer (ET) and Artificial Insemination (AI) Technologies as Tools Toward Achieving the Desired Number of Genetically Superior Breeder Dairy Cattle visited key facilities in Victoria, Australia last November 10-15, including the artificial insemination (AI) laboratory and stud farm at the head office of Genetics Australia; AgriBio Centre at La Trobe University; and Dairy Australia Southbank.

During the visit, the team learned the basics of rectal palpation from Mr. Brad Pickford at his training center and farm in Byuduk, Victoria. Mr. Pickford developed the Breed’n Betsy model (see image below), an artificial cow simulator device, which is a valuable aid for hands-on training on AI and embryo transer (ET).

Mr. Pickford using the Breed'n Betsy model he developed for large ruminant palpation training.
Training- rectal palpation in live animals.
DTRI reps palpating the reproductive tract of dairy cows at the training farm.

The team also participated in a lecture and demonstration at the in vitro fertilization (IVF) laboratory of Dr. Richard Fry at Speedbreed Donor Centre in Gnarawarre, Victoria.

The activity was conducted under Project 1 headed by Dr. Amado A. Angeles of UPLB-DTRI, and is a part of the PDRC Dairy Enhancement Program funded by the Department of Science and Technology through one of its agencies, the Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD).

PDRC Project 4 benchmarks somatic cell in milk of dairy cows in Batangas

The DTRI Project 4 team, led by Dr. Demetrio B. Marcial Jr., initiated in October 2019 the testing of raw milk in three dairy cattle farms in Tanauan and Lipa City, Batangas using the ImmunoCell California Mastitis Test (CMT) kits. This activity is part of the Philippine Dairy Research Consortium (PDRC) Project 4 on the Development of Farm-specific Protocols for the Reduction of Subclinical Mastitis in a Dairy Enterprise. It aims to establish the baseline for the somatic cell level of milk in cooperating farms.

The CMT is a qualitative cow-side test, which can be used by farmers to determine the somatic cell count (SCC) in raw milk of individual cows and the bulk milk prior to selling to processors.  The SCC in milk is an indication of on-going bacterial infection and may be used as a tool to diagnose subclinical mastitis cases in the farm.

Testing milk samples with CMT reagent. Gel formation indicates high SCC count.

The DTRI Project 4 is funded by DOST-PCAARRD through the Philippine Dairy Research Consortium (PDRC) Dairy Enhancement Program entitled Assisted Reproduction, Nutrition, and Health Interventions For Enhanced Dairy Cattle Productivity and Milk Safety. It aims to create specific management protocols to reduce subclinical mastitis in dairy farms. The reduction in subclinical mastitis should eventually increase the overall milk production of cooperating farms. The project started in December 2018 and will run for three years until the end of November 2021.

Effects of Using Frozen and Blast Frozen Raw Milk on the Quality of Dairy Products

Researchers : O.C. Emata, I.G. Sarmago and Ma. C.R. Moreno

ABSTRACT

SUBSTUDY 1: Effects of cooling, freezing, and blast freezing on the quality of raw cow’s milk. Emata, O.C. & M.C.R. Moreno.
The objective of the study was to compare the chemical, foaming, microbiological, and sensory qualities of cooled, frozen, and blast frozen raw cow’s milk. Raw cow’s milk was subjected to different storage conditions and treatments before analyses. Milk samples were cooled at 4°C for 24 hr (T1), frozen at 0°C for 24 hr and thawed at 4°C for 48 hr (T2), and blast frozen at -40°C for 10 hr and thawed at 4°C for 72 hr (T3). Fat, protein, solids-not-fat, and density were significantly different among treatments while moisture, total solids, titratable acidity, and pH did not
significantly differ. There were no significant differences in the foam value and foam volume between cooled and blast frozen raw milk. Foam dissipation was not significantly different among the treatments. The aerobic count was significantly lower in blast frozen raw milk. Coliform and Escherichia coli counts were not significantly different among the treatments. Sensory characteristics did not significantly differ among treatments except for flavor whereas frozen milk was significantly higher. In conclusion, freezing and blast freezing of raw milk has no negative effect on the chemical, foaming, microbiological, and sensory qualities of raw cow’s milk.
SUBSTUDY 2: Non-fat kefir production using kefir starter culture in cooled, frozen, and blast frozen cow’s milk. Emata, O.C., M.C.R. Moreno & I.G. Sarmago.
Kefir is a viscous, fermented, and carbonated dairy beverage produced from the association of lactic acid bacteria, acetic acid bacteria, and yeast of the kefir starter culture inoculated in milk. Commercial production of this beverage uses kefir starter culture due to difficulty in maintenance of kefir grains. Hence, the objective of the study was to compare the physicochemical qualities of kefir produced from inoculation of kefir starter culture in pasteurized and non-fat cooled (T1), frozen (T2), and blast frozen (T3) cow’s milk. Moisture, total solids, titratable acidity, and viscosity were significantly different among treatments. Results showed that kefir made from frozen and blast frozen milk had significantly lower moisture content and higher total solids content compared to kefir made from cooled milk. Kefir made from blast frozen milk did not significantly differ in titratable acidity from the two treatments. Kefir made from frozen and blast frozen milk were found to be significantly higher in viscosity. High total solids, efficiency of lactic acid production, and viscosity are some indicators of a high-quality kefir. Thus, utilization of frozen and blast frozen cow’s milk are suitable in kefir production.
SUBSTUDY 3: Production of half-and-half cream and light whipping cream using cooled, frozen, and blast frozen cow’s milk. Emata, O.C. & M.C.R. Moreno.
The objective of the study was to compare the physicochemical qualities of half-and-half and whipping creams made from cooled, frozen, and blast frozen cow’s milk. Raw cow’s milk was subjected to different cooling, freezing, and thawing conditions prior to cream processing. Half- and-half and light whipping creams were both produced from cooled (T1), frozen (T2), and blast frozen (T3) cow’s milk. Fat, moisture, total solids, titratable acidity, pH, and viscosity were significantly different among half- and-half cream treatments. All physicochemical components excluding fat did not significantly differ between half-and-half creams made from cooled
and blast frozen cow’s milk. On the other hand, titratable acidity was the only physicochemical component that was significantly different among light whipping cream treatments. However, all values were within the typical ranges for light whipping cream composition. In conclusion, the results indicate that cooled, frozen, and blast frozen cow’s milk were suitable for both half-and-half and light whipping cream productions.

Philippine Local Forages as Sustainable Feed Alternative for Dairy Cattle

Researchers : MM Loresco, JB Banayo, JML Roxas, KMD Saballo, RRCS Yambao, J Centeno, and AA Angeles

Funding Agency: USAID-STRIDE

Collaborator/s or Partner/s: Batangas State University – Lobo Campus, De La Salle Lipa, Benacorn Corporation, Philippine Asian Biotech Research and Development Corp. (PABRD), Samahan ng Maggagatas sa Batangas Cooperative (SAMABACO), Provincial Veterinary Office of Siquijor

ABSTRACT

Forages are very important component of the ration of ruminants (cattle, buffaloes, goats and sheep). They consume 60-80% roughage in their diet. Theprovisionofqualityforagestodairycattleisimportanttomeetthe nutrient requirement, which translates to high milk production. Moreover, forages provide the cheapest feed resource in the ruminant’s daily diet.
Nutrition provided to milking cows greatly affects its milk production as well as milk quality. With the downward trend in supply and quality of available roughage in the Philippines, there is a continuous effort to introduce new species of forage that would meet the demand of the country’s growing dairy industry.
The history of forage introductions in the Philippines started in the 1960’s when the Bureau of Animal Industry of the Department of Agriculture and the Dairy Training and Research Institute in UP Los Baños (UPLB) independently or through their joint-projects conducted researches on evaluation of forage accessions from Australia and other countries to spearhead pasture development and support the growing ruminant population in the country.
Improved forage species produce high quantity of herbage with regular application of fertilizers and efficient management (i.e. weed control, irrigation and controlled grazing). However, with continued use, improper management and exposure to adverse conditions, improved forages lose in competition with native species and this would necessitate re-establishment, a practice that is often very expensive.
On the other hand, indigenous plants have been dominant species in native vegetation surviving in equilibrium with the physical and biotic factors prevailing in the different agro-ecological zones throughout the country. They persist alongside introduced forages and they are also well relished by grazing animals. More so, they can tolerate extreme environmental conditions (i.e. drought and heavy rainfall) that are being experienced especially in recent years due to climate change. These indigenous plants such as trees, shrubs, vines and grasses can be utilized to partly replace the roughage requirement of ruminants especially in backyard farms.
To date, DTRI- UPLB has a collection of 40 introduced forages species. Some of these forages were introduced in the different regions in the country and became an important component of improved pasture for ruminant production. From this collection, six select forages were identified for use in the project. These forages are the common species grown in improved pastures for ruminant production, particularly in dairy and beef cattle farming.
Napier (Pennisetum purpureum), is a tall, high-yielding tropical grass, which is popular in cut-and-carry system of utilization. Its hybrid counterpart (Pennisetum purpureum x P. glaucum) produces more yield and has greater potential as livestock feed and as biofuel source. There are 6 ADSC accessions of hybrid Napier available for project use.
Guinea grass (Panicum maximum), a versatile bunch forming grass utilized for both cut-and-carry and grazing can perform well under partially shaded conditions. It is a well-adapted under the different agroecosystems in the country.
Signal (Brachiaria decumbens), and Jarra (Digitaria milanjiana) grasses were introduced more recently; Signal grass is suitable in marginal field conditions e.g. acidic and low soil fertility while Jarra grass has high nutritive value as shown by its high non-fiber carbohydrates (NFC) content.
Perennial Stylo (Stylosanthes guianensis), is herbaceous to shrubby type of forage legume with 16-18% crude protein content. It can provide additional protein to animals grazing grass pastures mixed with this legume. It also performs well under marginal conditions like acidic and low soil fertility.
We recognized the need to fully characterize these introduced forage species so that we can make proper recommendation to suit the local conditions and the dairy breeds being used in the Philippines.
The project reintroduced the six forages in selected regional areas of Batangas and Siquijor through the research and extension/training components of the project in collaboration with our project partners, Batangas State University, De La Salle Lipa, Philippine Asian Biotechnology Research and Development Corporation, Benacorn Corp. Provincial Veterinary Office Siquijor and Samahan ng Maggagatas ng Batangas Cooperative. The collaboration with Batangas State University Lobo Campus enabled us to conduct forage performance evaluation, establish a duplicate forage genebank and conduct a trainor’s training on forage production and conservation, activities which will have an impact in the revival of interest on local pasture resources. Batangas State University can replicate the training in their area by using the training module and manual developed for their use in educating the small farmers on the importance of quality forages as a cheap source of feed.
The forage introduction in Siquijor in collaboration with the Provincial Veterinary Office of Siquijor also provided a strong support system to the cattle genomic project, also being supported by USAID STRIDE, which tapped the local cattle population of the island as potential source of a local dairy breed in the country.
A tissue culture laboratory facility at DTRI-UPLB was established for the micropropagation of hybrid Napier grass. The successful propagation of Napier grass from leaf tissues via tissue culture can be considered as a pioneering work in the Philippines. This accomplishment will have important applications with regards to rapid multiplication of planting materials from the limited samples of elite forages available locally. The availability of enough quantity of planting materials is a vital support to the pasture development initiatives of both public and private sectors to sustain the growing ruminant industry in the country.
The forage performance evaluation conducted in three locations affirmed the significant effect of environment i.e. soil and climate on the herbage yield of the six select forages. The genetic potential of forages to produce high biomass was hindered by the unfavorable environment e.g. infertile soil, lack of water in the location where these were introduced. Long established concept of genetic x environment interaction on crop performance would necessitate the application of location –specific cultural management interventions to optimize the performance of the select forages. In the present study, the same cultural management practices such as fertilizer application was applied in all three locations to show the genotypic differences among the six forage species as expressed phenotypically through parameters like plant height, herbage yield and nutrient composition.
TMR feeding study provided results that lean towards the claimed advantage of feeding this complete dairy ration to milking cows. More milk of better quality i.e. high protein, minerals and total solids was produced by cows fed the more nutritious TMR (HD-TMR) compared with the high forage TMR (LD-TMR), however at the expense of lowered fat content. Higher number of experimental animals and replication of the feeding trial in backyard-, semi- and commercial- dairy farms would be necessary to obtain more valid results that would merit its commercial viability to dairy farmer-adopters.
The physiological effects that will be inferred through bioinformatics from the sequencing data of RNA extracted from milk of experimental animals was not determined in the present study due to the unexpected deterioration of RNA sample sent to the USA. Nevertheless, milk yield and quality data were considered sufficient to establish the beneficial effects of feeding high quality ration such as the TMR at the animal production level. We will consider pursuing this laboratory procedure in our future researches.
The project generated data and information from the numerous R & D activities conducted during its 15 months duration that could encourage our dairy farmers to reconsider the sustainable use of the local forage
resources that we currently have, and explore the utilization of indigenous plants as feed source to sustain animal production.
The project was able to create an impact to the communities that serve as hosts in the capacity building /extension work. This effort will be more effective when our partner educational institutions and LGU’s institutionalize the technology interventions and learnings in their programs. Batangas State University and De La Salle Lipa will be able to use the research results in relevant courses and also extend technologies to farmers through the conduct of regular training courses. Appropriate techniques in pasture establishment and management and forage preservation through ensiling were demonstrated to the select groups of farmers in Siquijor and Tanauan, Batangas during the project period. Dairy farmers in Batangas, due to its close proximity to UPLB, have close association with our agency with regards to our extension program e.g. training, technical assistance on dairy production and pasture development.
In the future, the TMR feeding study can be pilot tested in farms with enough number of milking cows (n=6 – 8) to validate the results obtained using a minimal number of cows in this study.

Mass Propagation of Hybrid Napier (Pennisetum Purpureum X P. Glaucum) by Callus Induction and Plantlet Regeneration from Leaf Segments

Researchers : Saballo, K.M.D., J.M.Roxas, M.M.Loresco, & J.B.Banayo.

ABSTRACT

In the Philippines, hybrid napier has shown itself to be well adapted to local climate and soil conditions. Though hybrid napier possess good quality traits for forage production, it does not produce seeds because it is sterile. This limits its potential for large scale production of planting materials and even future genetic improvement. The aim of this study is to develop the micropropagation protocol for hybrid napier by callus induction and subsequent plantlet regeneration. Shoots of veriety “Florida”were inoculated into callus induction media. The explants were cultured in MS nutrient media with 5% coconut water and varying concentrations of plant growth regulators. Callus formation was observed in as high NAA. Up to 46% of the explants formed big calli. Lower concentrations gave mostly medium sized calli 35-36%. Multiple plantlet formation was observed from the callus material regardless of size. We have demonstrated here that hybrid napier can be induced to form calli of varying sizes in as short as one month. This procedure is ideal for the mass propagation of elite line of hybrid napier.

Herbage Dry Matter Yield and Nutritive Composition of Mulato II (Brachiaria Hybrid) and Mombasa (Panicum Maximum Var. Mombasa) and Close Relative Grass Species in Pure Stand and as Mixture with Arachis and Centrosema

Researchers : M.M. Loresco, M.J.C. Andal, J.E. Opeña and A.A. Angeles.

ABSTRACT

The availability of forage species with high herbage yield and feeding value is important to sustain ruminant production. To address this concern, an in-house evaluation of two newly-introduced improved grasses, Mulato II and Mombasa, was conducted. The herbage dry matter yield and crude protein content of these two grasses and their close relative species, Signal and Guinea in pure stand and as mixture with Arachis and Centrosema were determined at two cutting intervals. Mulato II and Mombasa in pure stand produced higher herbage dry matter yield at the 30-
day cutting interval than their relative grass species. When mixed with either Arachis or Centrosema, Mulato II produced lower yield than Signal while Mombasa outperformed Guinea. Higher crude protein (CP) was measured in Mulato II than Signal in pure stand for both cutting intervals while lower CP was obtained in pure stand of Mombasa compared to Guinea. Overall, Mulato II had the highest crude protein content while Mombasa produced the highest herbage dry matter yield both in pure stand and when mixed with a forage legume. There was lower herbage yield in grass-legume mixtures compared with the pure grass as significant space was occupied by the forage legume, and which produced only low herbage yield at the early phase of evaluation.